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Publications

Articles

  1. Yoko Mizuta*, Masako Igarashi, Tomomi Shinagawa, Ikuma Kaneshiro, Haoto Anan, Daisuke Kurihara (2025) In vitro live cell imaging reveals nuclear dynamics and role of the cytoskeleton during asymmetric division of pollen mitosis I in Nicotiana benthamiana. Cytoskeleton 0:1-13.

    DOI: 10.1002/cm.70003

  2. Yoko Mizuta*, Daigo Sakakibara, Shiori Nagahara, Ikuma Kaneshiro, Takuya T. Nagae, Daisuke Kurihara, Tetsuya Higashiyama (2024) Deep imaging revealed dynamics and signaling in one-to-one pollen tube guidance. EMBO Rep 25:2529-2549.（Cover issue）

    DOI: 10.1038/s44319-024-00151-4  [Press release]  [Feature article]

  3. Naoya Sugi*, Daichi Susaki, Yoko Mizuta, Tetsu Kinoshita, Daisuke Maruyama (2024) Blue light irradiation induces pollen tube rupture in various flowering plants. Plant Cell Physiol. （Cover issue）

    DOI: pcae018. DOI: 10.1093/pcp/pcae018  [Press release]

  4. Ikuma Kaneshiro, Masako Igarashi, Tetsuya Higashiyama, Yoko Mizuta* (2022) Target pollen isolation using automated infrared laser-mediated cell disruption. Quant Plant Biol 3.

    DOI: 10.1017/qpb.2022.24

  5. Yoko Niimi, Keisuke Nagai, Motoyuki Ashikari, Yoko Mizuta* (2022) Deep fluorescence observation in rice shoots via clearing technology. J Vis Exp 184.

    DOI: 10.3791/64116  [Feature article]

  6. Daisuke Kurihara*, Yoko Mizuta, Shiori Nagahara, Yoshikatsu Sato, Tetsuya Higashiyama (2022) Optical clearing of plant tissues for fluorescence imaging. J Vis Exp 179.

    DOI: 10.3791/63428-v

  7. Daisuke Kurihara*, Yoko Mizuta, Shiori Nagahara, Tetsuya Higashiyama (2021) ClearSeeAlpha: Advanced optical clearing for whole-plant imaging. Plant Cell Physiol 62:1302-1310.

    DOI: 10.1093/pcp/pcab033

  8. Shiori Nagahara, Tetsuya Higashiyama, Yoko Mizuta* (2021) Detection of a biolistic delivery of fluorescent markers and CRISPR/Cas9 to the pollen tube. Plant Reprod 34:191-205.

    DOI: 10.1007/s00497-021-00418-z

  9. Nozomi Kawamoto, Dunia Pino Del Carpio, Alexander Hofmann, Yoko Mizuta, Daisuke Kurihara, Tetsuya Higashiyama, Naoyuki Uchida, Keiko U. Torii, Lucia Colombo, Georg Groth, Rüdiger Simon*. (2020) A peptide pair coordinates regular ovule initiation patterns with seed number and fruit size. Curr Biol 30:4352-4361.

    DOI: 10.1016/j.cub.2020.08.050

  10. Yoko Mizuta*, Katsutoshi Tsuda (2018) Three-dimensional multiphoton imaging of transcription factor by ClearSee. Method mol biol 1830:257-268.（Cover issue）

    DOI: 10.1007/978-1-4939-8657-6_15

  11. Yoko Mizuta*, Daisuke Kurihara, Tetsuya Higashiyama (2016) Visualization of plant sexual reproduction in the whole-mount pistil by Clearsee. Cytologia 81:1-2.（Cover issue）

    DOI: 10.1508/Cytologia.81.1

  12. Daisuke Kurihara*, Yoko Mizuta, Yoshikatsu Sato, Tetsuya Higashiyama (2015) ClearSee: a rapid optical clearing reagent for whole-plant fluorescence imaging. Development 142:4168-4179.（Cover issue）

    DOI: 10.1242/dev.127613  [Press release]

  13. Yoko Mizuta*, Daisuke Kurihara, Tetsuya Higashiyama (2015) Two-photon imaging with longer wavelength excitation in intact Arabidopsis tissues. Protoplasma 252: 1231-1240.

    DOI: 10.1007/s00709-014-0754-5

  14. Yoko Mizuta*, Tetsuya Higashiyama (2014) Antisense gene inhibition by phosphorothioate antisense oligonucleotide in Arabidopsis pollen tubes. Plant J 78: 516-526.

    DOI: 10.1111/tpj.12461  [Press release]

  15. Mitsuhiro Horade, Naoki Yanagisawa, Yoko Mizuta, Tetsuya Higashiyama, Hideyuki Arata* (2014) Growth assay of individual pollen tubes arrayed by microchannel device. Microelectron Eng 118: 25-28.

    DOI: 10.1016/j.mee.2014.01.017

  16. Mitsuhiro Horade, Yoko Mizuta, Noritada Kaji, Tetsuya Higashiyama, Hideyuki Arata* (2012) Plant-on-a-chip microfluidic-system for quantitative analysis of pollen tube guidance by signaling molecule: Towards cell-to-cell communication study. MicroTAS 2012. 1027-1029.

    https://www.rsc.org/images/loc/2012/pdf/T.2.58.pdf

  17. Masahiro Fujita, Youko Horiuchi, Yayoi Ueda, Yoko Mizuta, Takahiko Kubo, Kentaro Yano, Shinichiro Yamaki, Katsutoshi Tsuda, Toshihumi Nagata, Mitsuru Niihama, Hirotaka Kato, Shunsuke Kikuchi, Kazuki Hamada, Takako Mochizuki, Takeshi Ishimizu, Hiroaki Iwai, Nobuhiro Tsutsumi, Nori Kurata* (2010) Rice expression atlas in reproductive development. Plant Cell Physiol 51: 2060-2081.

    DOI: 10.1093/pcp/pcq165

  18. Yoko Mizuta, Yoshiaki Harushima, Nori Kurata* (2010) Rice pollen hybrid incompatibility caused by reciprocal gene loss of duplicated genes. Proc Natl Acad Sci U.S.A 107:20417-20422.（Cover issue）

    DOI: 10.1073/pnas.1003124107

  19. Junji Amano, Daisuke Nakazawa, Sachiko Kuwayama, Yoko Mizuta, Hajime Okuno, Yusuke Watanabe, Toshinari Godo, Dong-Sheng Han, Masaru Nakano* (2009) Intergeneric hybridization among colchicaceous ornamentals, Gloriosa spp., Littonia modesta and Sandersonia aurantiaca via ovule culture. Plant Biotechnol 26: 535-541.

    DOI: 10.5511/plantbiotechnology.26.535

  20. Keita Suwabe, Go Suzuki, Hirokazu Takahashi, Katsuhiro Shiono, Makoto Endo, Kentaro Yano, Masahiro Fujita, Hiromi Masuko, Hiroshi Saito, Tomoaki Fujioka, Fumi Kaneko, Tomohiko Kazama, Yoko Mizuta, Makiko Kawagishi-Kobayashi, Nobuhiro Tsutsumi, Nori Kurata, Mikio Nakazono, Masao Watanabe* (2008) Separated transcriptomes of male gametophyte and tapetum in rice: validity of a laser microdissection (LM) microarray. Plant Cell Physiol 49: 1407-16.

    DOI: 10.1093/pcp/pcn124

  21. Tokunori Hobo*, Keita Suwabe, Koichiro Aya, Go Suzuki, Kentaro Yano, Takeshi Ishimizu, Masahiro Fujita, Shunsuke Kikuchi, Kazuki Hamada, Masumi Miyano, Tomoaki Fujioka, Fumi Kaneko, Tomohiko Kazama, Yoko Mizuta, Hirokazu Takahashi, Katsuhiro Shiono, Mikio Nakazono, Nobuhiro Tsutsumi, Yoshiaki Nagamura, Nori Kurata, Masao Watanabe, Makoto Matsuoka (2008) Various spatiotemporal expression profiles of anther-expressed genes in rice. Plant Cell Physiol 49:1417-28.

    DOI: 10.1093/pcp/pcn128

  22. Junji Amano, Sachiko Kuwayama, Yoko Mizuta, Masaru Nakano* (2008) Morphological characterization of three intergeneric hybrids among Gloriosa superba ‘Lutea’, Littonia modesta, and Sandersonia aurantiaca (Colchicaceae). HortSci 43:115-118.

    DOI: 10.21273/HORTSCI.43.1.115

  23. Junji Amano, Sachiko Kuwayama, Yoko Mizuta, Tomo Oomiya, Toru Nakamura, Masaru Nakano* (2007) Early identification of intra- and intergeneric hybrids among colchicaceous ornamentals, Gloriosa spp., Littonia modesta Hook. and Sandersonia aurantiaca Hook., by flow cytometry and random amplified polymorphic DNA analyses. J Japan Soc Hort Sci 76: 73-78.

    DOI: 10.2503/jjshs.76.73

Cover
Collection

EMBO Rep
EMBO Rep
EMBO Rep
EMBO Rep
EMBO Rep
EMBO Rep
EMBO Rep
EMBO Rep

Review

  1. Mizuta Yoko* (2025) Mechanism of one-to-one pollen tube attraction revealed by deep two-photon imaging. Plant Morph 37:67-72. (Cover issue)

  2. Mizuta Yoko* (2023) Visualization of pollen and genome editing by transient transfection. Frontiers in Plant Science (BSJ-Review) 14:21-29.

    DOI: 10.24480/bsj-review.14a4.00239

  3. Yoko Mizuta* (2021) Advances in two-photon imaging in plants. Plant Cell Physiol 62:1224-1230.

    DOI: 10.1093/pcp/pcab062

  4. Yoko Mizuta*, Tetsuya Higashiyama (2018) Chemical signaling for pollen tube guidance at a glance. J Cell Sci 131:jcs208447.

    DOI: 10.1242/jcs.208447

  5. Kurihara, D.*, Mizuta, Y. (2017) Recommendations for fluorescent observation of plant tissues using clearing techniques. Plant Morph 29:81-86.

    DOI: 10.5685/plmorphol.29.81 (Cover issue)

  6. Mizuta Y, Kurihara D* (2014) Deep imaging and optical micromanipulation of living tissue using two-photon excitation microscopy. Plant Growth Regulation 49:96-103.

    DOI: 10.18978/jscrp.49.2_96

  7. Mizuta Y*, Kurihara D, Higashiyama T (2014) In vivo imaging of deep plant tissues using two-photon microscopy. Plant Morph 26:25-30.

    DOI: 10.5685/plmorphol.26.25

Book

  1. Science View Biological Resources, New Edition, The Scientist "The Ingenious Mechanism of Plant Fertilization" Jikkyo Publishing, Feb 2026 (ISBN: 9784407367959)

  2. Genome Editing Technology: Experimental Points/R&D Trends and Safety Information for Industrial Use (Contributor, Scope: Section 3, Paragraph 1, Delivery of Genome Editing Enzymes and Plant Characterization Improvement), Johokiko Co., Ltd., Jan 23, 2023 (ISBN: 9784865022421)

  3. Essential Genetics and Genomics (7th Edition) Chapter 9, Minako Ueda, Daisuke Kurihara, Yoko Mizuta (Co-translator, Coverage: Chapter 9, Original: Daniel L. Hartl, Chiharu Nakamura, Kiyotaka Okada), Kagaku Dojin, Feb 2021 (ISBN: 9784759820485)

特許

Industrial Property Rights

  1. Method for introducing substances into androgen cells (P2024-145393A)

  2. Method for concentrating target cells (2022-023027)

  3. A method for producing a carrier carrying microparticles coated with a substance to be introduced into cells, used in particle bombardment (P2021-132547A, P7526396)

  4. Method for introducing substances into pollen ( P2020-171262A, P7334898)

  5. Plant genome editing method (P2019-180373A, P6614622)

Other Activities

  1. "It's shiny! Is it transparent? Let's look inside the flower"
    Toyohashi City Audiovisual Education Center, Nagoya University, Nagoya University Outreach Lecture, December 7, 2025

  2. "Flowers Connect Science and Art"
    TEDxNagoyaU 2025 Round and Round (TED xTalks) May 5, 2025

  3. "Live-Cell Imaging of Plant Fertilization Using Nikon Multiphoton Microscope System"
    Nikon Solutions Co., Ltd. Application Note April 2025

  4. "Dive into the flowers. Unravel the mechanisms of life and discover its secrets!"
    FromPage Co., Ltd. Yume Navi August 2024

  5. "Two-photon imaging of plant reproductive processes"
    Coherent Japan Co., Ltd. and Nikon Solutions Corporation Multiphoton Microscopy Latest Imaging Seminar September 28, 2023

  6. "Automatic isolation of pollen using NIS-Elements General Analysis (GA) and JOBS imaging workflow tools"
    Nikon Solutions Co., Ltd. Application Note 2023

  7. Special Exhibision Plants, Mainstays of the Planet "Colorful pollen tubes in the pistil connect life to the next generation"
    National Museum of Nature and Science, NHK, NHK Promotion, Asahi Shimbun Co., Ltd. July 10, 2021 - April 3, 2022

  8. "Let's make it glow! Let's see! Experience being a scientist with fluorescent proteins"
    Sakae Common-S. Matsuzakaya Elementary School 13th Kids Science November 13, 2021

  9. "The Mystery of Flowers: The Hidden Mechanism of Life"
    Sakae Common-S. Nagoya University Cafe "Science, and Me" April 22, 2021

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